MS-Compatible magnetic IP kit, streptavidin, Pierce™

Supplier: Thermo Scientific
MS-Compatible magnetic IP kit, streptavidin, Pierce™
Protein Linking and Labeling Systems
The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS.

  • Directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MS
  • Antibody biotinylation reduces contamination in the eluate when compared to other IP methods

Uses high-quality Pierce streptavidin magnetic beads and optimisd buffers that are compatible with downstream LC-MS sample preparation and analysis. After the immunoprecipitation procedure, the target-enriched elution fraction is directly compatible with in-solution tryptic digestion, eliminating the need for gel purification, detergent removal, or desalting. This kit has been rigorously validated using numerous target antigens with varying expression levels, including targets previously undetected without enrichment or by western blotting. Store in refrigerator (2 to 8 °C).

Delivery information: The kit contains streptavidin magnetic beads (1 ml), IP-MS cell lysis buffer (100 ml), IP-MS wash buffer A (75 ml), IP-MS wash buffer B (40 ml), IP-MS elution buffer (6 ml).

Caution: For Research Use Only. Not for use in diagnostic procedures.
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