The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.
- Formulated to be compatible with downstream LC-MS analysis
- Binding, wash, and elution buffers optimized to minimize enrichment of background proteins
This kit has been rigorously validated using numerous target antigens with varying expression levels, including targets previously undetected without enrichment or by western blotting. Additionally, the reagents and procedures have been validated using both manual and automated magnetic separation.
Used for enrichment of low abundance targets to improve MS sensitivity Identification of target proteins that are not detectable by western blotting Quantitation of target proteins by LC-MS analysis Screening of antibodies for IP-MS Enrichment of target proteins upstream of targeted analysis (SRM).
Delivery information: The kit contains protein A/G magnetic beads (1 ml), IP-MS cell lysis buffer (100 ml), IP-MS wash buffer A (75 ml), IP-MS wash buffer B (40 ml), IP-MS elution buffer (6 ml), MgCl2 (1 M), (0,75 ml). Store in refrigerator (2 to 8 °C).
Caution: For Research Use Only. Not for use in diagnostic procedures.
