HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) ≥99.5%

Supplier: VWR Chemicals

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acidHEPES free acid

441476LEA 37654 INR
441476L
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) ≥99.5%
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

Hepes is a widely used buffer in biological studies. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10 to 15 mM).

Formula: C₈H₁₈N₂O₄S
MW: 238,31 g/mol
Melting Pt: 210…215 °C
Density: 1,448 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
Merck Index: 13,04672
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Specification Test Results

Assay Min. 99.5 %
Cl (Chloride) Max. 0.05 %
SO4 (Sulphate) Max. 0.05 %
Heavy metals (as Pb) Max. 10 ppm
pH (25°C; 1 %) 4.7 - 6.0
Water Max. 0.5 %

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