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HEPES, free acid, high purity

Supplier: VWR Chemicals

HEPES is a general purpose buffer available for biological research.

Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation and CO₂ fixation. Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for glutamate binding assays, preventing binding to non receptor materials.

Formula: C₈H₁₈N₂O₄S
MW: 238,31 g/mol
Melting Pt: 210-215 °C
Density: 1,448 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
UN: 0000
Merck Index: 13,04672
Specification Test Results
DNase none detected
Heavy Metals (as Pb) ≤0.0005%
Iron ≤0.0005%
pKa @20 °C 7.45 – 7.65
Protease none detected
Purity (Dry Basis) >99%
Residue on Ignition <0.2%
RNase none detected



Expand All / Collapse All
Pack type Pk VWR Catalog Number Quantity
HDPE Bottle 50 g 0511-50G
HDPE Bottle 250 g 0511-250G
HDPE Bottle 1 kg 0511-1KG
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