BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
- Packs are pouches of dry-blend powder that are each sufficient to make 500 ml of buffer
- Eliminates weighing time and pH adjustments
- Every pack will yield the same, consistent buffer
- Eliminates concerns about long-term stability of stock solutions
BupH™ Phosphate-Buffered Saline Packs are pouches of dry-blend powder that make sodium phosphate buffered saline (PBS) for use in crosslinking and labeling reactions requiring amine-free conditions. Each BupH dry-blend pack makes ready-to-use PBS with 0,1 M sodium phosphate, 0,15 M NaCl buffer at pH 7,2 when dissolved in 500 ml of water.
BupH MES-Buffered Saline Packs are pouches of dry-blended powder that make optimized amine-free and carboxyl-free buffer. When dissolved in 500 ml of water, the buffer contains 0,1 M 2-(N-morpholino) ethanesulfonic acid (MES), 0,9% sodium chloride at pH 4,7 for carbodiimide (EDC) crosslinking and conjugation reactions.
BupH Carbonate-Bicarbonate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 ml of alkaline (pH 9,4) sodium-carbonate (carb-bicarb) buffer. This buffer is used for coating or binding capture-antibodies and other proteins onto polystyrene microplates for ELISA and other plate assays. When dissolved in 500 ml of water, each pack makes 0,2 M carbonate-bicarbonate at pH 9,4.
BupH Tris-Buffered Saline Packs are pouches of dry-blend powder that make Tris-NaCl buffered saline (TBS) for diluents and wash buffers in ELISA, western blotting, and general immunoassay procedures. Each pack makes ready-to-use TBS with 25 mm Tris, 0,15 M NaCl at pH 7,2 when dissolved in 500 ml of water.
BupH Borate Buffer Packs are pouches of dry-blended powder that are each sufficient to make borate buffer for use in protein modification procedures requiring amine-free conditions. When dissolved in 500 ml of water, each pack makes 50 mM borate at pH 8,5.
BupH Tris-Glycine Transfer Buffer Packs are pouches of dry-blend powder that make 500 ml of standard transfer buffer for wet or semi-dry electrophoretic protein transfer from gel to blotting membrane. Each pack makes 25 mm Tris, 192 mM glycine at pH 8 when dissolved in 400 ml of water and 100 ml methanol.
BupH Modified Dulbecco's PBS Packs are pouches of dry-blend powder that make physiological sodium- and potassium-phosphate buffered saline (D-PBS) for ELISA and western blot diluents and wash buffers. Each pack makes 8 mm sodium phosphate, 2 mm potassium phosphate, 0,14 M NaCl, 10 mm KCl modified Dulbecco's PBS buffer at pH 7,4 when dissolved in 500 ml of water.
Caution: For research use only. Not for use in diagnostic procedures.
