The Pierce™ Primary Neuron Isolation Kit enables the optimal yield and viability of primary neurons from embryonic cortical and hippocampal tissue from mouse and rat.
- Procedure and reagents optimised for viability, yield, purity, and ease-of-use
- Provides two fold increase in yield compared to other methods
- Greater than 95% of isolated cells remain viable in culture from Day 1 to Week 4
- Cultured neurons processes indicative of complete differentiation and functionality
- Used in neuronal cell differentiation, immunohistochemistry functional and biochemical assays
The Primary Neuron Isolation Kit has been designed to obtain high viability and purity of primary embryonic neurons using validated reagents and an optimised procedure for enzymatic digestion and culturing. The kit contains tissue-specific dissociation enzymes, media formulated specifically for neuronal cell culture, and cell culture supplements that promote the isolation and growth of functional neurons. The validated protocol and reagents produce highly pure primary neurons for either short- or long-term culture, and the kit is designed for use by both experienced and non-experienced users.
Delivery information: Kit includes Neuronal Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), Neuronal Culture Medium, Neuronal Culture Media Supplement, Glutamine Supplement, and Neuronal Growth Supplement. Also required (but not supplied) are fresh embryonic mouse or rat tissue sample, heat-inactivated foetal bovine serum (FBS), and poly-D-lysine treated culture dishes.
