Amine reactive biotinylation reagents, EZ-Link™Supplier: Thermo Scientific
PIERA39257EA 0 INRPIER21328 PIER21336 PIER21312 PIER21326 PIER21313 PIER21335 PIER21330 PIER21362 PIER21217 PIER21219 PIER21441 PIER21343 PIER21331 PIER21329 PIERA39257 PIER21218 PIER21442 PIER21363 PIER20217 PIER21338
Amine reactive biotinylation reagents, EZ-Link™
Protein Linking and Labeling Systems
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
NHS-esters of biotin are water-insoluble and membrane-permeable because they do not possess a charged group. They may be used for biotinylating internal as well as external cellular components. Since NHS-esters of biotin are water-insoluble, for reactions in aqueous solution they must first be dissolved in an organic solvent, such as DMSO and DMF.
Sulfo-NHS-esters are water soluble and useful for applications that cannot tolerate organic solvents. Sulfo-NHS-esters of biotin are recommended for use as cell surface biotinylation reagents. Because of the charged sulfonate group, these compounds do not penetrate the plasma membrane, thus sulfo-NHS-esters biotinylation is restricted to the cell surface.
PEGylated esters transfer water solubility to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimise steric hindrance for binding to avidin molecules.