VWR®, Ready one-step RT-PCR kit

Supplier: Avantor

1B1326-50RXNEA 28201 INR
1B1326-50RXN
VWR®, Ready one-step RT-PCR kit
Nucleic Acid Reagents RT-PCR and cDNA Reagents
Ready One-Step RT-PCR Kit offers a single step procedure for performing RT-PCR reactions. Subsequent loading and visualisation of RT-PCR products are streamlined since the loading buffer and visualisation dye are included. The user supplies the primers and the DNase-treated template RNA.

  • One-step cDNA synthesis
  • Load directly to gel
  • Convenient format
  • Immediate visualisation after electrophoresis
  • Safe alternative to ethidium bromide

Once amplification is complete, the PCR reaction can be directly loaded and separated on an agarose gel using the magenta-colored tracking dye (migrating at approximately 10 bp on a 1% gel). After electrophoresis the PCR products are immediately visualised with standard UV illumination without additional post-run staining and destaining steps.

Delivery information: Ready One-Step RT-PCR kit includes Ready One-Step reverse transcriptase and Ready One-Step RT-PCR mix, 2X. Ready One-Step reverse transcriptase consists of M-MLV and a proprietary buffer that delivers robust cDNA synthesis from rare and abundant transcripts. Ready One-Step RT-PCR Mix, 2X consists of a mixture of reaction buffer, VWR® Extender™ Taq polymerase blend, dNTPs, an electrophoresis tracking dye, and a non-mutagenic EZ-Vision® visualization dye.
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