Ready One-Step RT-PCR Kit offers a single step procedure for performing RT-PCR reactions. Subsequent loading and visualisation of RT-PCR products are streamlined since the loading buffer and visualisation dye are included. The user supplies the primers and the DNase-treated template RNA.
- One-step cDNA synthesis
- Load directly to gel
- Convenient format
- Immediate visualisation after electrophoresis
- Safe alternative to ethidium bromide
Once amplification is complete, the PCR reaction can be directly loaded and separated on an agarose gel using the magenta-colored tracking dye (migrating at approximately 10 bp on a 1% gel). After electrophoresis the PCR products are immediately visualised with standard UV illumination without additional post-run staining and destaining steps.
Delivery information: Ready One-Step RT-PCR kit includes Ready One-Step reverse transcriptase and Ready One-Step RT-PCR mix, 2X. Ready One-Step reverse transcriptase consists of M-MLV and a proprietary buffer that delivers robust cDNA synthesis from rare and abundant transcripts. Ready One-Step RT-PCR Mix, 2X consists of a mixture of reaction buffer, VWR® Extender™ Taq polymerase blend, dNTPs, an electrophoresis tracking dye, and a non-mutagenic EZ-Vision® visualization dye.