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Single-stranded DNA binding protein (SSB)

Supplier: Affymetrix

Source: E. coli strain containing an overproducing clone of E. coli SSB protein.

Single-stranded DNA binding protein binds with high affinity in a cooperative manner to single-stranded DNA and does not bind well to double-stranded DNA. It is involved in DNA replication and recombination in vivo. This protein has been used to visualise single-stranded DNA by electron microscopy. It has also been used in conjunction with RecA protein for carrying out site-directed mutagenesis and to select sequences from libraries of double-stranded DNA. SSB may also stimulate specific DNA polymerases used in DNA sequencing reactions and has been used to target restriction endonuclease digestions to specific sites in single-stranded DNA for subsequent mutagenesis. SSB has been shown to be effective in fluorescence polarisation assays and eliminates pausing when sequencing through strong secondary structure. More recently, SSB was used to help obtain longer read lengths in pyrosequencing for SNP analysis.

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Description Pk VWR Catalog Number Quantity
Single stranded binding protein 500 µG USBM70032Z500UG
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