E.Z.N.A.® Endo-Free Plasmid Mini Kit IISupplier: Omega Bio-Tek
D6950-02EA 51699 INRD6950-02 D6950-01 D6950-00
E.Z.N.A.® Endo-Free Plasmid Mini Kit II
Nucleic Acid Purification Systems and Kits Plasmid Purification Systems and Kits
Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid Mini Kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0.1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.
- Endotoxins efficiently reduced (<0,1 EU/µg)
- No resin, slurries, or alcohol precipitation
- Fast, easy and convenient protocols