The E.Z.N.A.® HP Plasmid Mini Kit II is designed to isolate up to 30 µg of high-quality plasmid DNA from 5 to 10 ml bacterial cultures from strains that are endonuclease positive. The kit includes a protease digestion step to remove endonucleases and proteins from the sample. Plasmid DNA purification is simplified with HiBind® mini column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing.
- Purification of plasmid DNA in 30 minutes or less
- No phenol/chloroform extractions
- DNA is suitable for downstream applications