VWR® Silver Bullit™, Silver staining kit

Supplier: Avantor
VWR® Silver Bullit™
M227-1L-KITEA 32641 INR
M227-1L-KIT
VWR® Silver Bullit™, Silver staining kit
Stains Electrophoresis Stains
Silver Bullit™ Silver stain kit can be used to detect proteins in polyacrylamide gels with high sensitivity and nearly undetectable background. The kit's colorimetric staining procedure allows detection of sub-nanogram levels of protein, which is 100-fold more sensitive than Coomassie® blue staining.

  • Ultra-sensitive protein detection with clear background
  • Liquid concentrates for convenient reagent preparation

The staining procedure begins with a fixation step to reduce band diffusion and remove interfering substances and is followed by a sensitisation process, which enhances binding of silver ions to proteins. The staining step allows silver ions to permeate the gel and bind preferentially to sulfhydryl and carboxyl side chains. In the final development step, silver ions are reduced to metallic silver to form precipitates that are visible as brown bands within the gel.

Delivery information: Kit includes 10X silver stain, 250 ml; 10X sensitiser, 250 ml; 5X developer, 2×250 ml.
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